Stable Transfection for Long-Term Protein Production in CHO Cells
For applications requiring continuous and reliable protein expression, stable transfection offers decisive advantages. In a targeted process, we permanently integrate your gene of interest into the host cell genome. The result is a cell line that consistently produces protein in uniform quality and quantity over many passages – ideal for projects with high protein demand or long-term planning.
With our stable transfection services, you benefit from:
- Long-term protein production – stable expression across many cell passages
- High cost-efficiency – particularly economical for large-scale manufacturing
- Comprehensive project support – including expression optimization, cloning, and selection
Our expertise lies in the development of stable CHO cell lines manufactured according to ISO 13485 standards. This provides a secure foundation for consistent supply of biological raw materials – for example, for preclinical studies, diagnostics, or industrial applications.
Your Advantages with Stably Transfected Cell Lines from ASKA Biotech
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Permanent gene integration – consistently high expression without the need for re-transfection
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Reproducible results – essential for standardized processes and studies
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Reliable supply – ideal for continuous or scaled protein production
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ISO 13485-compliant development – ensuring regulatory safety for diagnostics and research
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Tailored process support – from construct design to finalized cell line
Your Stable Transfection Project – Let’s Talk!
Do you require long-term stable protein expression for your application? Then get in touch with us. We will advise you on the ideal expression strategy, take care of cloning, selection, and characterization, and develop a stable cell line that meets the highest quality standards – efficient, reproducible, and ISO 13485 certified.
Stable transfection is particularly suitable for the production of recombinant antibodies and recombinant proteins in larger quantities and with consistent quality.
Need rapid expression for short-term applications? Our transient transfection services offer flexible solutions for screening, bioassays, or preclinical studies.
FAQ – Frequently Asked Questions about Stable Transfection
Which cell systems do you use for stable transfection?
We primarily use CHO cells, which are especially suitable for the establishment of stable cell lines. Upon request, we can evaluate alternative cell lines for special requirements.
How long does it take to develop a stable cell line?
Establishing a stably transfected cell line usually takes up to 12 months. This includes transfection, selection, cloning, and extensive functional testing to ensure long-term stability.
Do you also offer protein production after transfection?
Yes, we also handle protein production – from small test batches to preclinical-scale manufacturing. Purification and quality control are already integrated into the process.
Is stable transfection suitable for IVDR applications?
Absolutely – our processes are ISO 13485 certified and can be adapted to comply with IVDR requirements, e.g., for the production of stable cell lines used as IVD raw materials.
Do you assist with expression optimization and clone selection?
Yes, our service includes the complete expression strategy – from expression optimization to the selection of the most productive clones.