Transient Transfection – Fast Protein Expression for Research & Development
Transient transfection offers a fast and flexible solution for short-term protein expression in HEK or CHO cells. Within just a few days, target genes can be efficiently expressed – without the need to establish a stable cell line. This makes it ideal for projects with tight timelines or lower protein demands.
Whether for feasibility studies, screening assays, functional analyses or test batch production: transient transfection enables you to quickly generate usable data – reliable, reproducible and ISO 13485-compliant.
Our experienced experts tailor the transfection process to your project goals and ensure an optimal balance of transfection efficiency, cell viability and protein quality.
Typical applications of transient transfection:
- Screening and expression analyses
- Antibody production in research scale (up to approx. 500 mg)
- Functional bioassays & prototype development
- Short-term material supply for preclinical studies
Your benefits with transient transfection at ASKA Biotech
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ISO 13485-compliant – high quality standards even in fast-track settings
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Very short project timelines – first results typically within a few days
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No need for stable cell lines – flexible and cost-efficient for small-scale production
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Versatile applications – ideal for screening, antibody testing, bioassays or pilot studies
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Compatible with HEK and CHO cells – depending on target protein and project scope
Your transient transfection project – get in touch with us!
Do you need proteins expressed quickly for screening, bioassays or preclinical studies? Then contact us. We’ll advise you on the optimal cell line and expression strategy, assist with vector design, and deliver high-quality results quickly – flexible, reliable and ISO 13485-compliant.
Transient transfection is also ideally suited for projects involving recombinant antibodies or recombinant proteins.
Looking for long-term expression instead? Our stable transfection services provide robust production systems – ideal for reproducible applications and continuous protein supply.
FAQ – Frequently Asked Questions on Transient Transfection
Which cell systems do you use for transient transfection?
We primarily use HEK and CHO cells for transient transfection. Both systems offer fast and efficient protein expression – we will advise you on the best choice for your project goals.
How quickly will I receive initial results?
You can typically expect expression data or protein samples just a few days after transfection – ideal for time-sensitive projects, feasibility studies or screenings.
How much protein can be produced via transient transfection?
Depending on the cell line and vector, we can produce up to approx. 500 mg of purified protein in laboratory scale – sufficient for many preclinical applications or assay development.
Can you meet IVDR requirements for transient production?
Our transient protein production is conducted under ISO 13485-compliant conditions. It is therefore suitable for IVDR-compliant validations or the production of diagnostic reagents.