Mammalian Antibody Expression Services (CHO/HEK) for Diagnostic & Research
If the DNA sequence of your target molecule is already defined, ASKA Biotech implements recombinant antibody manufacturing in specialized mammalian cell systems with maximum precision. Through our ISO 13485 validated processes, we guarantee complete documentation and batch-to-batch consistency for your IVD projects.
Depending on the project phase, we rely on rapid transient expression systems for fast prototyping or the generation of high-yield stable cell lines to ensure a long-term and scalable supply of your recombinant formats (rAbs).
Our Recombinant Antibody Expression Systems
We provide full flexibility in choosing the right expression system – based on your application, quality requirements, and required quantity:
CHO Cells (Chinese Hamster Ovary)
- The most commonly used system for industrial-scale antibody production. CHO cells enable reliable expression of full-length antibodies with human-like glycosylation.
- Ideal for diagnostic applications and preclinical studies. CHO cells also meet all regulatory requirements (e.g., IVDR).
HEK Cells (Human Embryonic Kidney)
- Optimal for transient transfection and high-throughput screening. HEK cells offer rapid expression of recombinant antibodies with excellent scalability.
- Especially suited for early-phase development, assay validation, and functional screening.
Recombinant Antibodies – Scalable Contract Manufacturing for Your Needs
As soon as your target molecule’s gene sequence is established, we handle the entire technical implementation up to the ready-to-ship product. Within our specialized contract manufacturing framework, we jointly select the optimal expression method to achieve your project goals efficiently: either through rapid transient expression for the fast generation of test batches or through the development of high-yield stable cell lines for maximum long-term stability and consistent antibody production.
This approach is ideal for:
- Complex Formats: Precise cloning and expression of humanized or chimeric antibodies with authentic glycosylation patterns.
- Specialized Fragments: Efficient recovery of smaller formats such as Fab fragments, scFv, or VHH (nanobodies) in high purity.
- Optimized Molecules: Engineering modified antibodies with specific tags (e.g., His, Strep) or optimized binding properties for demanding assays.
- Regulatory Projects: IVDR-compliant provision of recombinant antibodies for diagnostic development and large-scale preclinical studies.
Your Advantages with Our Recombinant Antibody Expression & Production
-
ISO 13485-Certified Processes – ideally suited for IVDR-compliant in vitro diagnostics and regulatory-sensitive applications.
-
Custom Process Development – we select the appropriate expression system (CHO or HEK) and tailor the workflow precisely to your project goals.
-
Flexible Batch Sizes – from small test batches to gram-scale production.
-
Experience & Expertise – benefit from our long-standing know-how in recombinant production of complex antibody formats.
-
Resource-Efficient Execution – optimized workflows and on-demand supply models help save time and costs.
-
Fast Turnaround – first results often available within 6-8 weeks thanks to high-performance expression systems.
Your Recombinant Antibody Project – Let’s Talk!
Are you planning to use recombinant antibodies in research, diagnostics, or preclinical development? Then you’ve come to the right place. Contact us today. Our expert team will support you with tailored advice, develop the optimal production strategy, and deliver reliable results – precisely, flexibly, and to the highest quality standards.
Do you rely on existing cell lines or need classical hybridoma manufacturing? Our monA service offers tailored solutions for this – from test production to scalable manufacturing.
Are you looking for the optimal expression host? We utilize rapid transient expression (HEK) for screening or stable CHO cell line development for high-yield production.
FAQ - Frequently Asked Questions about Recombinant Antibody Expression & Production
How long does recombinant antibody production take?
Our gene-to-antibody service typically takes 8-10 weeks. This timeline covers gene synthesis, vector construction, transient transfection (CHO/HEK), and purification. Urgent projects can be accelerated.
What quantities can be produced via recombinant expression?
We offer high-yield expression in CHO and HEK cells. Our scalable process covers everything from small pilot batches (mg scale) to bulk production (gram scale) for preclinical studies.
What quality controls do you perform?
We ensure maximum purity and activity. Standard QC includes CGE-SDS (purity), SEC-HPLC (aggregates), and A280 protein quantification. Optional assays include endotoxin testing (LAL) and functional ELISA.
Is your recombinant antibody manufacturing ISO 13485 certified?
Yes. As a specialized contract manufacturer in Germany, our recombinant antibody production is fully ISO 13485:2016 certified, ensuring the highest standards for IVD and diagnostic products.
Are your processes IVDR-compliant?
Yes, our production workflows are designed to be IVDR-compliant. We provide full documentation and traceability, making our antibodies suitable for regulatory-sensitive diagnostic applications.
In which format are the recombinant antibodies delivered?
Recombinant antibodies are typically delivered in liquid buffer formulations (e.g., PBS pH 7.4). We can provide custom concentrations, specific buffers, or lyophilized formats upon request.
Do you offer antibody engineering and modifications?
Yes, we offer extensive antibody engineering: from isotype switching and Fc-engineering to adding affinity tags (His/Strep) or conjugations with biotin, enzymes, and fluorophores.
How is shipping handled?
We ship worldwide via certified logistics partners. Depending on stability, shipments are cooled or frozen. We also offer dry ice shipping to ensure the integrity of your recombinant proteins.
Do you support project planning and vector design?
Yes, our support starts at the DNA level. We assist with codon optimization, vector design, and cloning strategies to maximize expression yields in the chosen system (HEK/CHO).
How is the pricing structured for recombinant services?
Pricing is based on project scope (e.g., gene synthesis, scale). We provide transparent quotes with a detailed cost breakdown from plasmid design to the final purified antibody batch.
Is a confidentiality agreement (NDA) possible?
Yes, we are happy to sign an NDA to ensure full protection of your sequence data and project information before we start.
