Stable Transfection – Custom Selection Strategies & Long-Term Protein Production in CHO
For applications requiring continuous and reliable protein expression, stable transfection offers decisive advantages. In a targeted process, we permanently integrate your gene of interest into the host cell genome. The result is a cell line that consistently produces protein in uniform quality and quantity over many passages – ideal for projects with high protein demand or long-term planning.
With our stable transfection services, you benefit from:
- Long-term protein production – stable expression across many cell passages
- High cost-efficiency – particularly economical for large-scale manufacturing
- Comprehensive project support – including expression optimization, cloning, and selection
Our expertise lies in the development of stable CHO cell lines manufactured according to ISO 13485 standards. This provides a secure foundation for consistent supply of biological raw materials – for example, for preclinical studies, IVDR-compliant diagnostics, or industrial applications.
Your Advantages with Stably Transfected Cell Lines from ASKA Biotech
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Permanent gene integration – consistently high expression without the need for re-transfection
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Reproducible results – essential for standardized processes and studies
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Reliable supply – ideal for continuous or scaled protein production
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ISO 13485-compliant development – ensuring regulatory safety and full IVDR readiness for diagnostics and research.
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Tailored process support – from construct design to finalized cell line
Your Stable Transfection Project – Let’s Talk!
Do you require long-term stable protein expression for your application? Then get in touch with us. We will advise you on the ideal expression strategy, take care of cloning, selection, and characterization, and develop a stable cell line that meets the highest quality standards – efficient, reproducible, and ISO 13485 certified.
Stable transfection is particularly suitable for the production of Recombinant Antibodies and Recombinant Proteins in larger quantities and with consistent quality.
Need rapid expression for short-term applications? Our Transient Transfection Services offer flexible solutions for screening, bioassays, or preclinical studies.
FAQ – Frequently Asked Questions about Stable Transfection & Cell Line Development
Which host cell lines do you use for stable cell line development?
We specialize in industry-standard mammalian systems, primarily CHO (Chinese Hamster Ovary). These are ideal for establishing high-yield stable cell lines with correct glycosylation.
What is the timeline for developing a stable cell line?
Developing a high-stability production clone is a precise process. It typically takes up to 12 months, covering vector construction, transfection, antibiotic selection, single-cell cloning, and stability testing.
Can you scale up from development to protein manufacturing?
Yes. As a full-service ISO 13485 contract manufacturer, we seamlessly transition from cell line development to protein production — from pilot batches to large-scale manufacturing.
Are your stable cell lines suitable for IVDR applications?
Absolutely. We develop stable cell lines under our ISO 13485 certified quality system in Germany. This ensures full documentation and traceability required for IVD raw materials and regulatory approval.
Do you assist with expression optimization and clone selection?
Yes, our service includes the complete workflow: from gene optimization and vector design to high-throughput screening for selecting the most productive and stable clones.