High-Yield Transient Expression in HEK & CHO within Days
Transient transfection offers a fast and flexible solution for short-term protein expression in HEK or CHO cells. Within just a few days, target genes can be efficiently expressed – without the need to establish a stable cell line. This makes it ideal for projects with tight timelines or lower protein demands.
Whether for feasibility studies, screening assays, functional analyses or test batch production: transient transfection enables you to quickly generate usable data – reliable, reproducible fully ISO 13485 & IVDR-compliant.
Our experienced experts tailor the transfection process to your project goals and ensure an optimal balance of transfection efficiency, cell viability and protein quality.
Typical applications of transient transfection:
- Screening and expression analyses
- Antibody production in research scale (up to approx. 500 mg)
- Functional bioassays & prototype development
- Short-term material supply for preclinical studies
Your benefits with transient transfection at ASKA Biotech
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ISO 13485-compliant – ensuring IVDR-compliant quality even in fast-track settings.”
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Very short project timelines – first results typically within a few days
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No need for stable cell lines – flexible and cost-efficient for small-scale production
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Versatile applications – ideal for screening, antibody testing, bioassays or pilot studies
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Compatible with HEK and CHO cells – depending on target protein and project scope
Your transient transfection project – get in touch with us!
Do you need proteins expressed quickly for screening, bioassays or preclinical studies? Then contact us. We’ll advise you on the optimal cell line and expression strategy, assist with vector design, and deliver high-quality results quickly – flexible, reliable and ISO 13485-compliant.
Transient transfection is also ideally suited for projects involving Recombinant Antibody Expression or Recombinant Protein Manufacturing.
Looking for long-term expression instead? Our Stable Transfection Services provide robust production systems – ideal for reproducible applications and continuous protein supply.
FAQ – Frequently Asked Questions on Transient Transfection
Which cell systems do you use for transient transfection services?
We specialize in mammalian expression systems. We primarily use HEK and CHO cells for transient transfection to ensure high protein yields and correct post-translational modifications.
How quickly will I receive results from transient transfection?
Speed is the main advantage of our transient transfection services. You can typically expect expression data or purified protein samples within a few days — ideal for rapid prototyping and feasibility studies.
How much protein can be produced via transient transfection?
Our transient protein production scale is flexible. Depending on the cell line and vector, we can produce up to approximately 500 mg of purified protein, sufficient for preclinical applications or assay development.
Can you meet IVDR requirements for transient protein production?
Yes. As an ISO 13485 certified contract manufacturer in Germany, our production is conducted under compliant conditions and can be validated to meet specific IVDR requirements for diagnostic reagents.